SANTA CRUZ:Vimentin Antibody (V9)

SANTA CRUZ:Vimentin Antibody (V9): sc-6260

• Vimentin Antibody (V9) is a mouse monoclonal IgG1 κ Vimentin antibody, cited in 1,158 publications, provided at 200 µg/ml

• raised against purified Vimentin from porcine eye lens

• Vimentin Antibody (V9) is recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; also reactive with additional species, including porcine

• Anti-Vimentin Antibody (V9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM

• also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems

• also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems

• also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM

• m-IgGκ BP-HRP is the preferred secondary detection reagent for Vimentin Antibody (V9) for WB and IHC(P) applications. This reagent is now offered in a bundle with Vimentin Antibody (V9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

维门丁抗体（V9）是一种针对猪眼晶体纯化的维门蛋白（主要表达于间充质细胞中的关键细胞骨架蛋白）培养的小鼠单克隆IgG1 κ轻链抗体。Vimentin小鼠单克隆抗体（V9）识别Vimentin免疫原序列中的表位，并在西部吸迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡嵌入切片的免疫组织化学（IHCP）和流式细胞术（FCM）应用中表现出广泛的物种交叉反应，包括小鼠、大鼠和人类样本。抗维门素单克隆抗体（V9）有非偶联形式和多种结合形式，包括辣根过氧化物酶、蓝藻酯、荧光素异硫氰酸酯、琼脂糖和各种Alexa氟®偶联物，具备多样化的实验需求。维曼素属于中间丝家族，能够保持细胞结构、完整性和机械韧性。维曼蛋白与细胞角蛋白、肌动蛋白和微管等多种蛋白质相互作用，形成复杂的细胞骨架纤维网络。维曼蛋白与运动蛋白的结合促进了细胞内运输和细胞器定位。虽然主要存在于细胞质内，但维氏蛋白在特定条件下会定位于细胞核和细胞膜。维门素是上皮向间充质过渡（EMT）的关键标志物，EMT是伤口愈合、发育和肿瘤转移的关键过程。维门素的表达模式在癌症生物学中尤为重要，因为维曼素在多种肿瘤中常表现出上调，包括黑色素瘤、肉瘤和乳腺癌，维曼素常与细胞角蛋白及其他中间丝共表达。Vimentin（V9）抗体使研究人员能够通过Vimentin在细胞质和膜染色的一致性特性，研究细胞骨架动力学、癌症进展及组织特异性表达模式。

Santa Cruz Biotechnology（简称 Santa Cruz，圣克鲁斯）是生物医学研究市场的核心参与者，总部位于美国德克萨斯州，自 1996 年成立以来，三十余年间始终专注于单克隆抗体、生物化学试剂、实验室器具及 CRISPR 基因编辑产品的研发与生产。

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SANTA CRUZ:Vimentin Antibody (V9): sc-6260